Extended Data Fig. 11: CD8⁺ cytotoxic assay and analysis of SARS-CoV-2 MA10 infection.

Ex vivo CD8⁺ cytotoxic assay where Her2⁺ mammary tumor cells were incubated with CD8⁺ cells enriched from lungs of WT, MMTV-Her2 mice, and MMTV-Her2 mice with CD4 depletion at 15 dpi (a) (n = 3/group). Ex vivo CD8⁺ cytotoxic assay where PyMT-expressing MET1 cells were incubated with CD8⁺ cells enriched from lungs of MMTV-Her2 mice with or without CD4 depletion 15 dpi (b) (n = 3/group). Concentrations of IFN-α, IFN-β, IFN-γ, IL-1β, and IL-6 in BAL at the indicated times post-MA10 infection (c, d). Quantification of Her2⁺ cells and percentage of Ki67⁺/Her2⁺ cells in lungs of MMTV-Her2 mice without (WT) or with IL-6KO 3 dpi (e) (n = 3 WT, 5 IL6KO). Infectious SARS-CoV-2 MA10 burden 3dpi with MA10 in lungs as quantified by plaque assay (f) (n = 6/group). Significance is determined by one-way ANOVA test (a,c,d) or two tailed Student’s t test (b,e). All box-and-whisker plots are presented as maximum value (top line), median value (middle line), minimum value (bottom line) with all data points shown (dots).

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