Fig. 3: IL-6 contributes to the awakening of dormant DCCs and proliferation.
From: Respiratory viral infections awaken metastatic breast cancer cells in lungs
a–c, Lung sections of MMTV-Her2 or IL-6 KO:MMTV-Her2 mice at 9 and 28 dpi with IAV (or PBS) were stained for HER2 (green) and DAPI (blue) (a) and quantified at 9 dpi (b) and 28 dpi (c). Scale bars: 50 μm. d, Quantification of the percentage of HER2+ Ki67+ cells in MMTV-Her2 and IL-6 knockout:MMTV-Her2 at 9 dpi (n = 4 per group). NS, not significant. e,f, Quantification of the percentage of vimentin+ HER2+ (e) and EpCAM+ HER2+ (f) cells in MMTV-Her2 and IL-6 knockout:MMTV-Her2 mice at 9 dpi (vimentin) and 3 dpi (EpCAM) (n = 3 for PBS, n = 4 IAV). Significance was determined by one-way ANOVA. g, Mean mammosphere area per well for HER2+ organoids after treating with 10 ng ml−1 IL-6 (n = 5 per group) (g); significance was calculated by Mann–Whitney test. All box-and-whisker plots are presented as maximum value (top line), median value (middle line) and minimum value (bottom line) with all data points shown by dots. All replicates are biological.
