Extended Data Fig. 1: Comparison of MMTV-Her2 and WT response to IAV infection and analysis of mammary glands, circulating blood.
From: Respiratory viral infections awaken metastatic breast cancer cells in lungs
Weight change of MMTV-Her2 and WT mice post-IAV infection, error bars denote standard deviation (a) (n = 3/group). Total cell counts in BALF 3, 6, 9, 15 dpi (b)(n = 3/group). IAV lung viral load at 3, 6, 9, 15 dpi or for vehicle control mice (c)(n = 3/group). There were no significant differences between BALF cell counts or IAV viral load between MMTV-Her2 and WT mice. Lungs of an 18-week-old mouse 28 dpi with IAV and a naïve 9-month-old mouse stain with Her2 (green) (d). IF detection of Her2 and Ki67 in mammary glands (e) and quantification of Ki67+ ducts 9 dpi (f) (n = 4/group). Ct values from real-time RT-PCR for transgenic rat Erbb2 mRNA in CD45neg cells in circulating blood from PBS or IAV infected MMTV-Her2 mice at 9 dpi (g). IF for p53 and Ki67 (with DAPI) (h) and quantification (i) for Ki67 of EO771 tumor lesions (2×105 EO771 cells implanted – left; 1 × 106 EO771 cells implanted - right) (n = 2/group). Significance is determined by one-way ANOVA test. All box-and-whisker plots are presented as maximum value (top line), median value (middle line), minimum value (bottom line) with all data points shown (dots).
