Extended Data Fig. 10: Lithium orotate restores spatial memory in J20 mice with advanced amyloid pathology.
From: Lithium deficiency and the onset of Alzheimer’s disease
a, Time course of spatial learning in the Morris water maze for J20 mice administered LiO (4.3 µEq/L) or vehicle (water) from 17–22 months of age. Shown are means ± S.E.M. b,c, Memory retrieval in the probe trial of the Morris water maze. Shown is the number of entries and time spent in the target area (b), and the latency to reach the target area (c). d,e, Swim speed and the latency to find a visible platform. f-h, LiO does not affect exploratory behavior in the open field test. Shown is distance travelled, distance traveled in the center of the arena, and the speed in the open field. b-h, Box plots show individual values, median (line), box limits (25th-75th percentiles), and whiskers (min-max). a, Learning data were analyzed using mixed-effects models with repeated measures, followed by Šídák’s post-hoc test. b-h, P-values by two-tailed unpaired Mann Whitney U test (b) or two-tailed unpaired t-tests (c-h). e, no significant differences were found. Vehicle n = 8 (b, left; c), n = 9 (a, b right, d-h); LiO n = 10 (a-e,g), n = 11 (f,h).
