Extended Data Fig. 1: Relationship to other pCCoV viruses.

Maximum likelihood tree of pantropic CCoV spikes (~450 bp region). Alignments were done with Muscle in MEGA7, and maximum likelihood tree was made in MEGA7 with default settings. Tree was visualized in iTOL. Extended Data Fig. 1 shows a maximum likelihood tree generated from the alignment of known pCCoV spike amplicons with the non-deletion form of the FCoV-23 amplicon. The alignment was carried out with Muscle in MEGA7 and the maximum likelihood tree was generated with MEGA7 on default settings. The tree was visualized with iTOL. The region targeted is ~450 bp with longer sequences trimmed down. The FCoV-23 spike with the deletion could not be used as the deleted region heavily overlaps with the region in the alignment. The FCoV-23 amplicon clusters among the pCCoV sequences.