Extended Data Fig. 12: IGF1 KO has minimal impact on iMG morphology, distribution and density.

a, Representative control and IGF1 KO microglial morphology visualized with IMARIS. b-d, Violin plots showing IGF1 KO does not significantly alter soma volume (b), total branch length (c), or branch number (d) in iMG. e-f, IHC images and bar graphs showing that IGF1 KO iMGs, like control iMGs, localize closer to NKX2.1⁺Ki-67⁺ proliferating progenitors than to NKX2.1⁺Ki-67^- cells in MGEOs. g-h, IHC images and bar graphs showing a high percentage of SOX2⁺ cells among Ki-67⁺ proliferating progenitors located near IGF1 KO iMGs, similar to control iMGs. i, iMG density in 6-week-old MGEOs is not significantly different among different IGF1 treatment and iMG genotyping conditions. Unpaired two-tailed t-test in (b)-(d); P value of interaction effect of distance x Ki-67 were labelled, two-way repeated measures ANOVA in (f); N = 4,5 in (h); N = 5 in (i), one-way ANOVA. Data in (b)-(d) were shown as violin plots with medians (solid line) and quartiles (dash line) indicated. Data in (f), (h), (i) were shown as means ± SEM.