Extended Data Fig. 15: Microglial IGF1 is not involved in MGE proliferation in mice.
From: Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1
a, IGF1 is expressed by ATM located at developing white matter at P5. b, IGF1 is expressed by ATM-like microglia located at cortico-striato-amygdalar boundary at E14.5. Arrows indicate IGF1+ microglia in a and b. c, IGF1 is not detectable in microglia located in the mouse MGE at E14.5. d, Pregnant dams received IP tamoxifen injections at E11.5 and E12.5, followed by an EdU injection at E14.5, four hours prior to fetal brain collection and subsequent IHC processing. e-f, IHC images and bar graph showing that microglial Igf1 cKO mice (Igf1f/f, Cx3cr1CreERt/+) show a comparable MGE proliferation to their littermate controls. N = 5 and 5 from three litters. Unpaired two-tailed t-test. Data in (f) were shown as means ± SEM. Illustration in d was created using BioRender (https://biorender.com).
