Fig. 5: Microglia-derived IGF1 promotes MGE proliferation in MGEOs.
From: Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1
a, Experimental schematics. b, IHC images indicating the specific expression of IGF1 in iMG of 6-week-old MGEOs. c, IHC images showing the expression of IGF1R in progenitors in the rosettes of 6-week-old MGEOs. d,e, IHC images (d) and bar graphs (e) showing that IGF1 promotes MGE progenitor proliferation in MGEOs without iMG to the level seen in MGEOs with iMG, and that IGF1-neutralizing antibody treatment abolished iMG-mediated progenitor proliferation. f,g, IHC images (f) and bar graphs (g) showing the inability of IGF1 knockout (KO) iMG to promote MGEO progenitor proliferation, which can be rescued by the addition of recombinant IGF1 treatment. h, Graphical abstract showing microglial regulation of interneuron progenitor proliferation through IGF1 in hMGE. For statistics, n = 10, 11 and 9 for MGEO −iMG; n = 10, 9 and 9 for MGEO +iMG (e). n = 3, 5, 5 and 5 (g). Two-way ANOVA (e), one-way ANOVA (g) and post hoc Bonferroni’s test for selected comparisons. Data in e and g are shown as mean ± s.e.m. Scale bars, 50 μm (b,c), 200 μm (d,f). Illustration in a was created using BioRender (https://biorender.com).
