Extended Data Fig. 8: CD20 targeted B cell depletion.
From: EBV induces CNS homing of B cells attracting inflammatory T cells
a-b, Weekly total numbers of CD45+CD19+ or CD20+ B cells in blood of EBV-infected mice with and without Rituximab treatment (PBS n = 5, EBV n = 4, Rituximab n = 7). p-values from left to right: CD20:0.0026, 0.0003,0.029,0.0120CD19: 0.0014,0.0004.0.0235,0.0110, (two-way mixed effects model with Geisser-Greenhouse correction with Tukey’s multiple comparisons test). Dots present mean and error bars SEM. c, Frequency and d, total numbers of CD19+ B cells in spleen (p-value < 0.0001) and brain with and without Rituximab treatment. e, Frequency of CD20+ B cells in spleen (p-value = 0.0007) and brain (p-value = 0.0011) with and without Rituximab treatment. f, Total numbers of CD20+ B cells in spleen (p-value = 0.0007) and brain (p-value = 0.0217) with and without Rituximab treatment. g, In vivo bioluminescence signal of perfused brains (PBS n = 5, EBV n = 4, Rituximab n = 7). h, Total numbers of CD19+CD11c+T-bet+CXCR3+ B cells in the blood (p-value < 0.0001), spleen (p-value = 0.0002), and brain (p-value = 0.0201) with and without Rituximab treatment (PBS n = 5, EBV n = 4, Rituximab n = 7). i, Total numbers of CD4+ T cells in the brain with and without Rituximab treatment (EBV n = 4, Rituximab n = 7). j, Correlation between activated HLA-DR+ CD4+ T cells and T-bet+CXCR3+ B cells in the brains of EBV infected humanized mice. Solid line represents trend line obtained by linear regression, the dotted lines indicate 95% confidence bands (n = 12). k, Total numbers of CD4+ T cell subtypes, including naïve (CD45Ra+CCR7+), effector memory (CD45Ra-CCR7-), central memory (CD45Ra-CCR7+) and Temra (CD45Ra+CCR7-) cells, with and without Rituximab treatment (EBV n = 4, Rituximab n = 7). a-k, were generated from 2 independent experiments with mice reconstituted from 2 different human CD34+ hematopoietic progenitor cell donors. Unless otherwise indicated, p-values were calculated using the two-tailed MWU test. Error bar indicates SEM; each dot represents one single animal. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
