Extended Data Fig. 2: Characteristics and origin of EBV expanded T-bet⁺CXCR3⁺ B cells.

a, Representative flow cytometric contour plots of T-bet⁺CXCR3⁺ and T-bet⁺CD11c⁺ B cell gating of splenocytes from PBS or EBV-infected humanized mice. b, Frequencies of CD19⁺CD11c⁺ B cells in blood, spleen and brains of PBS or EBV-infected humanized mice (PBS n = 21, EBV n = 28). c, Frequencies of CD19⁺CD11c⁺T-bet⁺ B cells in blood (p-value = 0.0277), spleen and brains of PBS or EBV-infected humanized mice (PBS n = 21, EBV n = 28). d, Total numbers of CD19⁺T-bet⁺CD11c⁺ B cells in blood, spleen and brains of PBS or EBV-infected humanized mice (PBS n = 21, EBV n = 28). e, Frequency of T-bet⁺CXCR3⁺ and T-bet^-CXCR3^- cells among CD11c⁺ B cells in blood (p-value = 0.0004), spleen (p-value < 0.0001) and brains of EBV infected mice. f, Frequency of T-bet⁺CXCR3⁺ and T-bet^-CXCR3^- cells among CD11c⁺ B cells in blood (p-value < 0.0001), spleen (p-value < 0.0001) and brains of PBS mice. g, In vitro EBV infection of CXCR3⁺ and CXCR3^- B cells monitoring T-bet/CXCR3 co-expression frequency over time (p-values from left to right: 0.0211, 0.0160, 0.0016, 0.0007, 0.0039, <0.0001). h, In vitro EBV infection of CXCR3⁺ and CXCR3^- B cells monitoring CD11c frequency over time (p-value = 0.0461). i, Growth assay of established LCLs derived from CXCR3⁺ or CXCR3^- B cells over time depicted as total numbers. g-i, p-values were calculated using mixed-effects model with Geisser-Greenhouse correction followed by Sidak’s multiple comparisons test. Unless otherwise indicated, p-values were calculated using the two-tailed MWU test. Data from figures b-f, was generated from 5 independent experiments with mice reconstituted from 6 different human CD34⁺ hematopoietic progenitor cells donors. Error bar indicates SEM; each dot represents one single animal. Data from Figure g-i was generated from 3 independent experiment using 3 B cells donors. Error bar indicates SEM; dots represent mean of all 3 donors. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.