Extended Data Fig. 4: Transcriptional characteristics of T-bet+CXCR3+ B cells.
From: EBV induces CNS homing of B cells attracting inflammatory T cells
a, UMAP of T-bet+CXCR3+ among splenic B cells of EBV-infected humanized mice by scRNA-seq. b-c, Stacked bar plots showing (left) the CD19+ B cell population frequency and (right) immunoglobulin isotype distribution within T-bet+CXCR3+ and non-T-bet+CXCR3+ (other) cells. d, Expression of indicated Ig isotypes and e, expression of indicated EBV transcripts. f, Heatmap of shared clones between B cell subtypes with Morisita overlap index. g-h, Violin plot of expression of MKI67 (adjusted p-value = 1.41e-260) and pro-proliferative gene module (MKI67, PCNA, TOP2A, CCNB1, CCNB2, CDK1; adjusted p-value = 2.64e-157) between infected (EBV+) and un-infected (EBV-) T-bet+CXCR3+ B cells. i-j, Violin plot of expression of MKI67 (adjusted p-value = 2.105e-86) and pro-proliferative gene module (MKI67, PCNA, TOP2A, CCNB1, CCNB2, CDK1; adjusted p-value = 2.86e-32) between clonally expanded and diverse BCR expressing T-bet+CXCR3+ B cells. k, Violin plot of expression of TBX21 between infected (EBV+) and un-infected (EBV-) CXCR3+ B cells (adjusted p value = 1.706e-29). l, IGHV gene expression among the top 50 expanded B cell clones. IGHV4-34 and IGHV4-39 expressing clones are highlighted. The black line defines the mean expression of each group from g-j. P-values for differential gene expression analysis between groups were calculated using the two-sided MWU test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
