Extended Data Fig. 2: RNA hydrolysis suppresses adventitious aminoacylation.
From: Thioester-mediated RNA aminoacylation and peptidyl-RNA synthesis in water
RNA hydrolysis proceeds by means of fragmentation, which yields 2′-phosphate and 3′-phosphate through 2′,3′-phosphate intermediate. The high pKa of these alcohols inhibits aminoacylation and prevents the adventitious aminoacylation of RNA degradation products. PAGE images show the reaction of ds-oligomeric RNA (0.5 µM) with aminoacyl-thiol l-\({{\bf{1}}}_{{\bf{A}}{\bf{l}}{\bf{a}}}^{{\bf{e}}}\) (200 mM, R = Me, R1 = Et) in MES buffer (200 mM, pH 6.5) and KCl (500 mM) after 16 h at room temperature: left, 5′-FAM-UAGGAGACCA (ON5) + 5′-GCAGUUGGUCUCCUA (ON7); right, 5′-FAM-UAGGAGACCA-3′p (ON5-3′p) + ON7.
