Extended Data Fig. 3: Synthesis of aminoacyl-thiols from activated amino acids.
From: Thioester-mediated RNA aminoacylation and peptidyl-RNA synthesis in water
a, Yield of aminoacyl-thiol \({{\bf{1}}}_{{\bf{Aaa}}}^{{\bf{c}}}\) from NCA (4) and coenzyme M (5c) in MES buffer (200 mM) at room temperature after 10–30 min. *Phosphate buffer. Glx = glutamine-γ-nitrile. For further data and other thiols (5), see Supplementary Figs. 132–151. b, The reaction of aminoacyl-adenylate 2 (20 mM) with thiol 5b (100 mM) in MES buffer (200 mM, pH 6.0) yields aminoacyl-thiol \({{\bf{1}}}_{{\bf{Ala}}}^{{\bf{b}}}\) (42%). For yield of \({{\bf{1}}}_{{\bf{Ala}}}^{{\bf{b}}}\) observed from reaction of aminoacyl-adenylate 2 and thiol 5b at alternative pHs (pH 2.0–5.0), see Supplementary Table 39.
