Extended Data Fig. 1: Inactivation of TGDS in 4 different cell lines does not affect most nucleotide sugar levels.
From: A missing enzyme-rescue metabolite as cause of a rare skeletal dysplasia
a, Sequence of the genomic locus and predicted TGDS proteins in TGDS-deficient HAP1, U2OS, 293 T and HCT116 cell line clones. b-g, CMP-sialic acid (b), GDP-mannose (c), UDP-hexose (d), UDP-GlcNac (e), GDP-fucose (f), and CDP-ribitol (g) were determined by LC-MS in parental cells and two TGDS KO clones from U2OS, HCT116, HAP1 and 293 T. Data present means ± SD of three (U2OS, HCT116 and 293 T) or four (HAP1) experiments performed with 3 independent samples each, and are normalized to levels observed in wild-type cells. * p < 0.05 in paired two-tailed Dunnett post-hoc testing of log-transformed data after one-way ANOVA. For exact p-values see source data file.
