Extended Data Fig. 10: A simplified methylation toolkit controls transposable elements in P. gotoi.
From: The genomic origin of the unique chaetognath body plan
a, Methylation is only found on transposon-derived sequences. a) Number of methylated genes in Paraspadella (mCG > 20%) that contains enriched Pfam domains (Two sided Fisher exact test p-value < 0.01). Only the top most abundant domains are shown, with all those known to be associated with retrotransposons shaded in red. b, Heatmap showing the ATAC-seq and Enzymatic Methyl-seq signal across all gene models, sorted by methylation intensity. c, Heatmap showing the methylation levels on the major types of repetitive elements in the Paraspadella genome. Only >500 bp repeats/inserts are displayed. Domain architecture of DNA methylation toolkit in Paraspadella. d, The domain architecture of DNMT1, UHRF1, TET and DNMT3 genes in an ancestral configuration and in P. gotoi, defined by PfamA domain presence/absence. RFD (PF12047), zf-CXXC (PF02008), BAH (PF01426), DNA_methylase (PF00145), TTD (PF12148), PHD (PF00628), SRA (PF02182), Ring Finger (PF00097), TET_JBP (PF12851). e, Expression of P. gotoi methylation toolkit in broad cell types.
