Extended Data Fig. 4: Limited compartimentalisation of P. gotoi chromatin.

a, Mapping statistics of the Hi-C library highlighting the number of mapped and inferred contacts used to generate contacts maps including those in cis- b, Resolution of Hi-C library estimated by computing the number of bins containing >1000 contacts at increasingly higher resolutions. c, Hi-C contacts map in the Hox cluster region showing insulation scores and putative TAD boundaries revealing the limited structuration of chromatin in the Hox region. d, TAD metaplot showing the limited domain structuration of P. gotoi chromatin (average within TADs observed/expected contact ratio = 1.05) as well as the expected pattern in case of strong TAD structuration shown as the bottom right inset. e, Saddle plot showing interactions strength across active and inactive regions with inset showing expected pattern for strong A/B compartmentalisation. f, Pearson correlation matrices and associated eigenvectors that best capture centromere position, calculated at 250 kb resolution for the 9 chromosomes of P. gotoi. Putative centromere positions are indicated (highest eigenvector value differential). Hi-C maps suggest a lack of A/B structures (except possibly for chr5).