Fig. 1: TCF1 and LEF1 are highly expressed in B-1 cells and B-1a cells are reduced in TCF1–LEF1-deficient mice.
From: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function
a, Gene expression in the different clusters projected on uniform manifold approximation and projection (UMAP) of scRNA-seq from total peritoneal B cells. b,c, Flow cytometry plots showing TCF1 or LEF1 expression (b) or both (c) in peritoneal cavity B cells. d, Flow cytometry plots showing gating strategy of B220low, B220intermediate and B220high cells from peritoneal cavity B cells, CD5 and PtC expression and histogram of TCF1 and LEF1. FMO, fluorescence minus one. e–g, Flow cytometry plots showing CD38+ and CD38− B cells, CD43, CD5, TCF1 and LEF1 expression from the indicated compartments (e,f) and quantification of the percentage of CD43+CD5+ cells within the CD19+ cells in healthy donor (HD; n = 7), CLL (n = 11), pleural infection (n = 3) and sleep disordered breathing/otitis media with effusion ((SDB/OME); n = 6; g). CTV, CellTrace Violet; FSC, forward scatter; PBMC, peripheral blood mononuclear cell; PF, pleural fluid. h, Contour plots (top) and quantification (bottom) showing PtC reactivity in the indicated subsets from freshly processed PBMC of HD (n = 3). i, Fold change of expression of TCF1 and LEF1 between CD43+CD5+ cells and CD43−CD5− cells in patients with CLL. j–m, Contour plots and quantification of the percentage of B-1, B-2, B-1a and B-1b cells from the peritoneal cavity (j,k); B-1 and follicular B cells from the spleen (l,m) from TCF1WTLEF1WT (n = 4 or 6), TCF1Δ (n = 4), LEF1Δ (n = 6) or TCF1ΔLEF1Δ (n = 4 or 5). n, Frequency of the indicated peritoneal and splenic B cell populations in sublethally irradiated Rag1−/− recipient mice 6 weeks after reconstitution with fetal embryonic day 14.5 liver cells from mice of TCF1WTLEF1WT (n = 4) or TCF1ΔLEF1Δ (n = 7). Each symbol represents an individual mouse and bars represent median values. Data are representative of n = 5 (e), n = 3 (k–m) and n = 4 (n) experiments. Data are from n = 5 (g) experiments and n = 3 donors (h). Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey multiple-comparison test (g,k,m) and two-tailed Mann–Whitney U-test (n) or two-way ANOVA (h). The exact P values are shown.
