Fig. 2: TCF1 and LEF1 are required for B-1a self-renewal.
From: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function
a,b, Contour plots (a) and quantification (b) of positive cells and TCF1 or LEF1 expression in peritoneal B-1a cells from 4 (n = 5), 8 (n = 4) and 16 (n = 3) weeks of age mice. c, Contour plots showing B-1a cells from TCF1WTLEF1WT and TCF1ΔLEF1Δ mice at 4 (top) and 14 (bottom) weeks of age, gated on peritoneal CD19+B220− cells (left) and quantification (right). d, Experimental diagram (top left), dot plots (right) and quantification (bottom left) of BrdU-labelled donor-derived B-1a or B-2 cells: TCF1WTLEF1WT (n = 3) and TCF1ΔLEF1Δ (n = 4). e,f, Circular plots show cell numbers of the different clonotypes (e) with corresponding heatmap (number of clones) and quantification of peritoneal B cell clones with a particular VH–VL pairing from TCF1WTLEF1WT and TCF1ΔLEF1Δ mice. Clones composed of Ighv11-2/Igkv14-126, Ighv12-3/Igkv4-91 and Ighv9-3/Iglv2 were labelled as c1, c2 and c3, respectively (f, top) and their frequency quantified (f, bottom). g, Contour plots (left) and quantification (right) of PtC liposome-binding CD5+ PC B-1 cell numbers from TCF1WTLEF1WT (n = 6) and TCF1ΔLEF1Δ (n = 4) mice. h, Quantification of the percentage of peritoneal and splenic B-1 cells from ETS1WT (Ets1+/+.CreCd19; n = 5) and ETS1Δ (Ets1flox/flox.CreCd19; n = 4) mice. Each symbol represents an individual mouse, and the bars indicate median values. Results are representative of n = 2 (a–d,g,h) experiments. Statistical analysis was performed using two-tailed Welch’s t-test (d), Mann–Whitney U-test (g,h), one-way ANOVA with Tukey multiple-comparison test (b) or two-way ANOVA (c). The exact P values are shown.
