Fig. 3: TCF1 and LEF1 in B-1a cell mitosis, development and gene expression.
From: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function
a, Gene set enrichment analysis of bulk RNA-seq from TCF1WTLEF1WT and TCF1ΔLEF1Δ B-1a cells shows enrichment for the indicated signalling pathways. The black dotted lines indicate neutral enrichment. b, Correlation analysis between expression of TCF1 or LEF1 and expression of MYC (top) or BCL2 (bottom) in peritoneal B-1a, B-1b and B-2 cells (n = 6). gMFI, geometric mean fluorescence intensity. c, UMAP of scRNA-seq profiles from the four indicated sorted cell subsets (FL B1P and BM B1P), B-1 cells from the spleen (SP B1) and peritoneal cavity (PC B1) barcoded and pooled for sequencing. d,e, Developmental trajectory of the combined (d) or individual (e) FL B1P, BM B1P, SP B1 and PC B1 populations constructed by Monocle2. f, Comb plots displaying the incidence and amplitude of the indicated genes in each subset shown in panel d. g, Dot plot presentation of hallmark gene sets upon gene set enrichment analysis for differentially expressed genes in B-1a cells of the indicated genotypes. h, Dot plot presentation of expression of the indicated genes in human prenatal B-1 cells according to single, double or no TCF1 and LEF1 expression. Panel h was adapted with permission from ref. 23, AAAS. Data are representative of n = 2 experiments. Statistical analysis was performed using two-tailed Pearson correlation analysis (b). The significant hallmark gene sets in MSigDB (adjusted P < 0.05) for each contrast based on the hypergeometric testing using cluster profiler package (g).
