Extended Data Fig. 4: TCF1 and LEF1 effect on BCR signalling of B-1 cells.
From: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function
(a) Histogram (top) and gMFI quantification (bottom) of phosphorylation (p-) status of various BCR signaling components: p-BLNK (TCF1WTLEF1WT n = 6; TCF1ΔLEF1Δ n = 4); p-Btk (TCF1WTLEF1WT n = 6; TCF1ΔLEF1Δ n = 4); p-PLCγ2 (TCF1WTLEF1WT n = 6; TCF1ΔLEF1Δ n = 4); p-Syk (TCF1WTLEF1WT n = 5; TCF1ΔLEF1Δ n = 7); p-ERK1/2 (TCF1WTLEF1WT n = 5; TCF1ΔLEF1Δ n = 7) in B-1a cells. (b) Flow cytometry plots (top) and gMFI (bottom) of Igκ and Igλ in B-1a and B-2 cells from TCF1WTLEF1WT (n = 7) and TCF1ΔLEF1Δ (n = 5). (c) Histogram (left) and gMFI (right) of CD19 in B-1P from foetal liver (TCF1WTLEF1WT n = 3; TCF1ΔLEF1Δ n = 5) and B-1a cells (TCF1WTLEF1WT n = 4; TCF1ΔLEF1Δ n = 4) from peritoneal cavity. Each symbol represents an individual mouse and bars represent median values. Data are representative of n = 2 experiments (a-c). Statistical analysis was performed using two-tailed Mann-Whitney t test (a,c), one way ANOVA with Tukey multiple-comparison test (b). The exact P values are shown.
