Extended Data Fig. 2: Transformation of sulfide during the incubation of D. alkaliphilus with ferrihydrite (ca. 62 mM) and periodic supply (n = 8) of a low concentration of sulfide (ca. 50 µM) at 1.5-h intervals.
From: Microbial iron oxide respiration coupled to sulfide oxidation
a, Development of sulfate, HCl-extractable Fe(II), elemental sulfur (S(0)), and Cline-extractable sulfide during the incubation experiment (n = 3 replicate cultures). Parallel incubations with living cells without Fh or sulfide, and with killed cells served as controls. The arrows in each panel indicate the addition of sulfide. The red stars denote sampling from the culture for chemical analysis. The inoculum used for the incubation experiment had an OD600 of 0.072. b, Kinetics of dissolved sulfide following the first three spikes to ferrihydrite-amended culture in a parallel incubation experiment with the same inoculum as in panel a. Sulfide measurements from two biological replicates are displayed. The data confirmed that the culture consumed sulfide more rapidly than the abiotic control. c, Schematic graph showing the flow of spiked sulfide during the 12-h incubation. Sulfur metabolite concentrations derive from data in panel a. All values are the means of triplicate cultures with standard deviation.
