Extended Data Fig. 3: snRNA-seq reveals CNS cell-type composition and microglial heterogeneity across disease states.
From: Microglia–neuron crosstalk through Hex–GM2–MGL2 maintains brain homeostasis
A) Selected marker genes associated with each cell type highlighted in Fig. 3a. B) Typical marker genes associated with each immune cell type highlighted in Fig. 3b. C) Dotplot depicting common microglial homeostatic and disease-associated genes among clusters shown in Fig. 3c. D) Heat map featuring the top cell-type-specific marker genes across the major cell types. The color bar indicates gene expression. E) Marimekko plot depicting the different cell type compositions separated by age or genotype. F) UMAP and Marimekko chart of microglia and CAMs depicting the proportions of Hexb−/− and Hexb+/− microglia for each cluster. G) UMAP visualization of microglia cluster from different conditions shown in Fig. 1a. Here, microglia from P120 Hexb−/− microglia are integrated. H) Marimekko plot depicting the cluster proportions for the indicated conditions. I) Heat map featuring the top cluster marker genes. Shared Hexb−/− disease genes are highlighted. J) UMAP visualization of microglia shown in g. Color code indicates their belonging to the homeostatic or disease condition. K) UMAP visualization of microglia shown in g. Color code indicates the respective condition.
