Extended Data Fig. 2: Hexb deficiency leads to microglial activation, astrogliosis, and axonal damage throughout the mouse brain with regional differences.
From: Microglia–neuron crosstalk through Hex–GM2–MGL2 maintains brain homeostasis
A) Development of body weight over disease course (left) and strength in the Grip Strength Test (right) for Hexb−/− (n = 15), Hexb+/− (n = 15), and Hexb+/+ (n = 15) controls. B) Measurement of blood marker for liver and kidney damage in Hexb+/− and Hexb−/− mice. One symbol indicates one biological replicate. C) TNF and IL-6 levels in the blood of Hexb+/− and Hexb−/− mice measured by ELISA. Paired t-test comparison was used for statistical testing. D) Quantification of APP (axonal damage), GFAP (astrocytosis), IBA1 (microgliosis), and Mac-3 (lysosomal microglia activation) in different brain regions (cortex, cerebellum grey and white matter, hippocampus, thalamus, and pons/medulla) at different time points (P0, P7, P28, P56, P85, P120) for Hexb−/− (n = 4) and Hexb+/− (n = 4) controls. E) Representative immunohistochemical image of IBA1/Mac-3 double-positive cells in the thalamus of P120 Hexb−/− mice. F) Representative immunofluorescence images indicating lysosomal activation in P7 Hexb−/− (orange) and Hexb+/− (blue) microglia. G) Left: Representative immunohistochemical images of P2RY12+ and TMEM119+ cells in the thalamus at P120Right: Quantification Each dot represents one individual mouse. Th: thalamus, Cwm: Cerebellum – white matter, P/M: pons/medulla, Cgm: Cerebellum – grey matter, Ctx: cortex, H: hippocampus. Data shown as mean ± s.e.m. Statistical analyses: Two-tailed Student’s t-test was used for statistical testing (B-C); Two-way ANOVA followed by Sidak’s multiple comparison test was used for statistical testing (D,G).
