Fig. 2: Assembly of NCLX.

a, The density map of an NCLX trimer. The density map (Ca²⁺ bound, cytosol-facing conformation) is coloured by subunit. b, The structure of an NCLX trimer. The Ca²⁺ bound, cytosol-facing conformation of NCLX is shown as a ribbon from the cytosolic and matrix sides, respectively, with TM11 and TM12 labelled at the trimer interfaces. c, The conformational state assembly of NCLX trimers. The transport domain is coloured cyan, whereas the gate domain of each protomer is coloured according to the conformational state. The conditions leading to the 3D reconstructions with specific conformational state assembly are indicated by the coloured lines below.