Extended Data Fig. 9: Neuron-conditioned media increases proliferation of SCLC, but not to the extent of direct co-culture.

a, Quantification of proliferative index of murine 16T SCLC cells exposed to conditioned media (CM) from or directly co-cultured with iPSC-derived glutamatergic neurons with or without addition of 1 µM TTX (n = 3 coverslips per condition, p < 0.0001). Conditioned media alone renders a partial increase in SCLC proliferation compared to baseline, but not to the full effect of direct co-culture (grey). b, As in a, but human H69 SCLC cells were used (n = 3–5 coverslips per condition, p = 0.0003). c, As in a, but iPSC-derived GABA-ergic neurons were used (n = 3–6 coverslips per condition, p < 0.0001). d, As in c, but human H69 SCLC cells were used (n = 3–4 coverslips per condition, p = 0.0007). e, Quantification of proliferative index of murine 16T SCLC cells exposed to conditioned media (CM) from lung epithelial cells (n = 3 coverslips per condition, p = 0.9010. f, Quantification of proliferative index of murine 16T SCLC cells at baseline or when treated with 100 nM neuroligin 3 (NLGN3) or 100 nM brain derived neurotrophic factor (BDNF). 10% FBS condition is used as positive control (n = 4 coverslips per condition, p = 0.9403 for NLGN3, p = 0.8969 for BDNF). g, As in f but for human H446 SCLC cells (n = 4 coverslips per condition, p = 0.9387 for NLGN3, p = 0.9969 for BDNF). h, Quantification of proliferative index of patient derived glioma cells (SU-DIPGVI) at baseline or treated with 100 nM NLGN3 (n = 4 coverslips per condition, p = 0.0483). i, as in h for glioma cells treated with 100 nM BDNF (n = 4 coverslips per condition, p = 0.0096). Data are mean ± s.e.m. Analysis with one-way ANOVA for a, b, c, d, f, g, two-tailed unpaired t-test e, h, i. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.