Fig. 3: Transcriptional response after knockdown of ChmA.
From: Programmable antisense oligomers for phage functional genomics
a, PAO1 cells were pretreated with 6 µM ASO targeting chmA for 30 min. Cells were infected with ΦKZ at an MOI of 5 and incubated for the indicated times post infection followed by RNA-seq. To reduce the dimensionality of the dataset, we projected the data based on transcript abundance of each annotated gene on two dimensions via principal components analysis. Each dot represents an independent experiment. Var., variance. b, Volcano plot of the log2-transformed fold change (log2(fold change)) of transcripts of samples treated with the ASO targeting chmA compared with the control ASO at the indicated time points. Two independent replicates were merged by geometrical averaging and P values were calculated by the Wald test (two-sided) using DESeq2.
