Fig. 1: Late-onset kidney inflammation in Mgme1−/− mice.
From: Ribonucleotide incorporation into mitochondrial DNA drives inflammation
a, Row-clustered heat map (log2-transformed fold change (FC)) of mRNA ISG profiles obtained by NanoString profiling in Mgme1+/+ and Mgme1−/− mouse kidneys at different ages. The asterisks mark ISGs of which the expression was significantly altered between Mgme1−/− and Mgme1+/+ mouse kidneys at 70 weeks of age. n = 2 mice per genotype at 10, 35 and 55 weeks and n = 3 mice per genotype at 70 weeks. b, Heat map (log2-transformed fold change, decreasing order) of mRNA ISG profiles obtained by NanoString profiling of various tissues of 55-week-old WT (Mgme1+/+) and Mgme1−/− mice. The asterisks mark ISGs of which the expression was significantly altered between Mgme1−/− and Mgme1+/+ mice in the corresponding tissue. n = 3 mice per genotype per tissue. c, dPCR analysis of mtDNA levels in cytosolic fractions of kidney (n = 6 mice per genotype) and liver (n = 3 mice per genotype) tissue from 55-week-old control (Mgme1+/+) and Mgme1−/− mice. A schematic of the mtDNA and of the probes used in the assay is shown at the top right. The blue dashed line represents the major arc, and the remaining part of mtDNA is minor arc. P values were calculated using unpaired two-tailed Student t-tests (c). Data are mean ± s.d. *q < 0.05, **q < 0.01 (a,b).
