Extended Data Fig. 4: Increased cytosolic cGAS localization in Mgme1−/− cells.
From: Ribonucleotide incorporation into mitochondrial DNA drives inflammation
a, cGAS localization in Mgme1+/+ and Mgme1−/− immortalized MEFs, monitored by confocal immunofluorescence imaging. Cells were stained with DAPI (blue), Phalloidin (green) and anti-cGAS antibody (red). Scale bars, 5 μm. b, Quantification of cGAS localization in the nucleus and the cytosol of Mgme1+/+ and Mgme1−/− immortalized MEFs obtained by confocal immunofluorescence analysis. cGAS nuclear mean intensity (left panel), cGAS cytosolic mean intensity (middle panel) and ratio of cytosolic to nuclear mean intensities (right panel) are shown. n = 51 and n = 64 independent Mgme1+/+ and Mgme1−/ cells were analysed, respectively. c, Immunoblot analysis of total and cytosolic fractions of Mgme1+/+ and Mgme1−/− MEFs used in Fig. 2c. A representative blot of three independent fractionation experiments is shown. P values were calculated using unpaired two-tailed Student t-te st (b). Data are presented as mean ± SD.
