Fig. 6: Increased ribonucleotide content of mtDNA in aged kidney and liver tissues.
From: Ribonucleotide incorporation into mitochondrial DNA drives inflammation
a, LC–MS analysis of the ratios (log2-transformed fold change) of rNTP and dNTP levels from kidney lysates of young (aged 1 week; n = 8 mice) and old (aged 80–96 weeks; n = 7 mice) mice. b, Untreated and RNase-H2-treated DNA isolated from kidney of young (aged 1 week) and old (aged 87 weeks) mice was analysed by alkaline agarose gels and Southern blotting. Quantification of Southern blots was performed as described in Fig. 4d. c, LC–MS analysis of the ratios (log2-transformed fold change) of rNTP and dNTP levels from liver lysates of young (aged 1 week; n = 8 animals) and old (aged 80–96 weeks; n = 6 animals) mice. d, The same as b, except that liver tissue was analysed. P values were calculated using unpaired two-tailed Student t-tests (a and c). Data are mean ± s.d.
