Extended Data Fig. 1: Increased ISG signalling in kidneys from Mgme1−/− mice.
From: Ribonucleotide incorporation into mitochondrial DNA drives inflammation
a, Agglomerative heat map showing the distribution of mRNA ISGs (upper panel) and non-ISGs (lower panel) intensities in Mgme1+/+ and Mgme1−/− mice kidneys at different ages. Z-score intensities were calculated and clustering was performed using the Euclidean distance metric and the ‘average’ method. Each column represents a different mouse. b, c, Heat map (log2 fold change, decreasing order) of non-ISG inflammation related mRNA profiles in Mgme1+/+ and Mgme1−/− kidneys at different ages (b) and in various tissues of 55-week-old mice (c) obtained by NanoString technologies. d, Heat map showing the distribution of mitochondrial proteins (MitoCarta 3.0, n = 503, quantified in all samples) in subcellular fractions of kidney lysates using the iBAQ intensity scaling the lowest and highest value between zero and one. The rows were clustered using the Euclidean distance metric and the ‘average’ method. Each column represents a different mouse (n = 6 mice per genotype). e, TFAM protein levels (log2 iBAQ intensity) in subcellular fractions of kidneys of 55-week-old Mgme1+/+ and Mgme1−/− mice, identified by quantitative proteomics. The boxplot is defined as follows: each box spans from the 25% to the 75% quantile and the line center indicates the median. The whiskers indicate the minimum and maximum excluding outliers. Outliers are determined by using 1.5 times the inter-quantile range (a cross indicates outliers). n = 6 animals. *q < 0.05, ** q < 0.01.
