Fig. 1: In vitro reconstitution and mTORC1 kinase activity with liposomes.
From: Structural basis for mTORC1 activation on the lysosomal membrane
a, Cartoon showing the in vitro membrane-containing reconstitution of mTORC1 kinase activity. N-terminal 6×His LAMTOR1 is tethered with DGS-NTA(Ni), and Cys181 of RHEB is lipidated by reaction with PE MCC. b, Determinants of mTORC1 activation. Western blot is shown for anti-phospho (p)-4E-BP1 (Thr37/Thr46). Activation fold change is computed relative to the activity of mTORC1 alone. Quantifications are indicated with bars from three experiments and are mean ± s.d. All samples were derived from the same experiment, and gels and blots were processed in parallel.
