Extended Data Fig. 4: The interaction between mLST8 and RagA.
From: Structural basis for mTORC1 activation on the lysosomal membrane
a, Cryo-EM density map (contour level 0.149) and model of mLST8-Rag-Ragulator subcomplex. b, Electrostatic surface potentials of mLST8 and RagA are shown. The interaction surfaces are outlined. c, Close-up view of the interaction between mLST8 and RagA. d, Overlay between GTP-bound and GDP-bound RagA (PDB: 6NZD), the missing interswitch region is indicated with arrow. The GDP-bound RagA has a disordered interswitch region, which is responsible for the interaction with mLST8. In addition, the α8 helix in the RagA-GDP has potential clash with mLST8.
