Extended Data Fig. 5: Hybridization chain reaction (HCR) analysis of hoxd13a expression in the fins of control and 5DOM deletion animals.
From: Co-option of an ancestral cloacal regulatory landscape during digit evolution
a. Maximum projection confocal images of 60 hpf pectoral fins from homozygous 5DOM deletion animals and wildtype siblings labeled for hoxd13a transcripts. Deletion fins express hoxd13a in the expected domain but at attenuated levels. b. Quantification of hoxd13a signal in wildtype and mutant fins (n = 7 each genotype). Relative intensity is normalized to the wildtype mean. While fin signal trended lower in mutant fins, this difference was not significant (Welch Two Sample t-test, p = 0.1553). The boxes represent the interquartile range (IQR), with the lower and upper hinges denoting the first and third quartiles (25th and 75th percentiles). Whiskers extend from the hinges to the furthest data points within 1.5 times the IQR. The upper whisker reaches the largest value within this range, while the lower whisker extends to the smallest value within 1.5 times the IQR from the hinge. Scale bar: 50 μm.
