Fig. 3: Hoxd gene expression in the mouse urogenital system.

a, Schematic representations of male and female urogenital systems. UGS is indicated with a red circle. b, WISH of Hoxd13 in representative female and male urogenital systems. Hoxd13 is selectively expressed in UGS. c, Schematic representation of the two HoxD inversion alleles. The locations of the inversion breakpoints are depicted with red arrows. Hox genes shown in shades of purple. d, Hoxd13 expression in urogenital systems of mice carrying the inversions (WISH, left panel; RT–qPCR, right panel). HoxD^{Inv(Itga6–attP}: wild type (n = 4 samples) and inversion (n = 3 samples); HoxD^{Inv(Itga6–nsi)d11lac)}: wild type (n = 3 samples) and inversion (n = 3 samples). Expression of Hoxd13 in UGS was abolished when the target genes were disconnected from 5DOM. The boxes represent the interquartile range (IQR), with the lower and upper hinges denoting the first and third quartiles (25th and 75th percentiles). Whiskers extended from the hinges to the furthest data points within 1.5 times the IQR. The upper whisker reached the largest value within this range, whereas the lower whisker extended to the smallest value within 1.5 times the IQR from the hinge. Each experiment was repeated independently at least twice with similar results (b,d). B, bladder; K, kidney; O, ovary; T, testis. Scale bar, 1 mm (b,d).