Extended Data Fig. 10: Macrophages from 12-week-old NOD mice and human islet leukocytes analysed by scRNASeq.
From: Efferocytic remodelling of pancreatic islet macrophages by limited β-cell death
a. scRNASeq analysis (UMAP plots) showing macrophage subsets from pancreatic islets at 12 weeks of age (late pre-diabetic) from NOD mice treated with STZ1-low or vehicle at 4 weeks of age (at early pre-diabetic stage). b. Markers of macrophage subsets from (a). c. Reanalysis of a publicly available dataset showing Igf1r expression across CD4+ T cell subsets from spleen and lymph node (GSE24427844). (Wald statistics, DESeq2). Data are presented as mean values ± SEM, 3 biological replicates over N = 1 RNASeq experiment. d. Flow cytometric analysis of human donor pancreatic islet cells. Islet macrophages were identified as Live, CD45+ CX3CR1+ CD11c+ cells. Representative of N = 2. e. Human islet leukocytes analysed by scRNASeq, 3 individual donors. CD45+ cells were FACS-purified as shown on (d), left. Transcriptional data was integrated between the donors and subjected to clustering. f,g. Markers differentially expressed between the identified immune cell types in human pancreatic islets. The diagram in d was created in BioRender. Ravichandran, K. (2025) https://BioRender.com/ddu5zzj.
