Extended Data Fig. 1: Efferocytosis by islet macrophages following STZ1-low treatment in C57BL/6 mice.
From: Efferocytic remodelling of pancreatic islet macrophages by limited β-cell death
a. Immunofluorescent confocal microscopy imaging showing confocal planes of the pancreatic islets of C57BL/6 mice 12 h after STZ1-low or control vehicle treatment. Green – apoptotic cells (cleaved caspase-3, CC3), blue – nuclei (Hoechst). Scale bar 30 µm. b. Quantification of fraction of apoptotic cells (CC3+) relative to the total cell number in the islets (Hoechst+). n = 9 islets in both conditions. c. Confocal images of islets taken 12 h after STZ1-low treatment showing X-Y, Y-Z, and X-Z views of efferocytic events. Red – insulin, white – macrophages (F4/80), green – apoptotic cells (CC3), blue – nuclei (Hoechst). The CC3/insulin double positive areas inside the macrophages are considered as efferocytic events. The top row of images corresponds to (Fig. 1b). Scale bar – 30 µm. d. Quantification of efferocytic events via Imaris software automatic counting (see Methods). n = 9(Vehicle), n = 8(STZ1-low). e. Percentage of islet macrophages (Live, CD45+, F4/80+, CD11c+) relative to all islet cells based on flow cytometric analysis 10 days after treatment with STZ1-low or vehicle (C57BL/6 mice). n = 8(Vehicle), n = 11(STZ1-low) mice. Statistics - two-tailed unpaired t-test, data shown as mean ± SEM.
