Extended Data Fig. 6: lPBN infusion of NPY suppresses persistent inflammatory and chronic pain.
From: A parabrachial hub for need-state control of enduring pain
(a) Neuropeptide Y (NPY) or vehicle was infused into the lPBN. Withdrawal thresholds were subsequently tested in animals with persistent inflammation from an injection of Complete Freund’s Adjuvant (CFA) in the paw or SNI-induced neuropathy. (b) Withdrawal threshold to mechanical stimuli before CFA (baseline) and after CFA with a vehicle or NPY infusion (n = 7 mice, one-way ANOVA, p < 0.001). (c) Paw volume before CFA injection (baseline) and after CFA with a vehicle or NPY infusion (n = 8 mice, one-way ANOVA, p < 0.001). (d-e) Withdrawal threshold to a cold stimulus (d, acetone) or mechanical stimuli (e) before SNI (baseline) and after SNI with a vehicle or NPY infusion (n = 7 mice, one-way ANOVA, p < 0.001). Data are expressed as mean ± SEM. Grey lines represent individual mice. Post-hoc comparisons: *p < 0.05, **p < 0.01, ***p < 0.001. ANOVA main effect of group: ###p < 0.001.
