Extended Data Fig. 7: Competing needs suppress persistent pain, but not acute pain.
From: A parabrachial hub for need-state control of enduring pain
(a) Time spent licking paw (5 min bins) after an injection of formalin in ad libitum-fed (n = 9) or 24 h food-deprived (n = 7) mice (two-way ANOVA, main effect of group p = 0.0031). (b) Time spent licking paw during phase 1 (n = 9 ad lib, n = 7 FD mice, unpaired two-sided t-test, n.s.). (c) Percent of trials where a paw withdrawal was observed after mechanical stimulation with a von Frey filament before CFA and after CFA in ad libitum-fed or food-deprived mice. Each von Frey filament was applied 5 times (n = 15 mice, two-way ANOVA, main effect of group p < 0.001, group x filament interaction p < 0.001). (d) Withdrawal threshold before SNI and after SNI in ad libitum-fed or food-deprived mice (n = 11 mice, one-way ANOVA, p = 0.0008). (e) Time spent licking paw (5 min bins) after an injection of formalin in mice with ad libitum access to water (n = 8) or 24 h water-deprived mice (n = 6) (two-way ANOVA, main effect of group p < 0.001). (f) Time spent licking paw during phase 1 (n = 8 ad lib, n = 6 WD mice, unpaired two-sided t-test, n.s.). (g) Percent of trials where a paw withdrawal was observed after mechanical stimulation with a von Frey filament before CFA and after CFA in mice with ad libitum access to water or water-deprived mice (n = 10 mice, two-way ANOVA, main effect of group p = 0.0005, group x filament interaction p = 0.0434). (h) Withdrawal threshold before SNI and after SNI in mice with ad libitum access to water or water-deprived mice (n = 11 mice, one-way ANOVA, p < 0.001). (i) Water intake 1 and 2 h after vehicle or PEG treatment (n = 10 mice, two-way ANOVA, main effect of group p = 0.0019). (j) Timeline of PEG experiments. (k) Time spent licking paw (5 min bins) after vehicle or PEG administration (n = 9 mice/group, two-way ANOVA, main effect of group p = 0.003). (l) Time spent licking during phase 1 (n = 9 mice/group, unpaired two-sided t-test, n.s.). (m) Time spent licking during phase 2 (n = 9 mice/group, unpaired two-sided t-test, p = 0.0043). (n) Percent of trials where a paw withdrawal was observed after stimulation with a von Frey filament before CFA and after CFA in vehicle- or PEG-treated mice (n = 8 mice, two-way ANOVA, main effect of group p < 0.001, group x filament interaction p = 0.0235). (o) Withdrawal threshold before CFA and after CFA in vehicle- or PEG-treated mice (n = 8 mice, one-way ANOVA, p = 0.0088). (p) Withdrawal threshold before SNI and after SNI in vehicle- or PEG-treated mice (n = 10 mice, one-way ANOVA, p < 0.001). (q) Withdrawal duration after acetone administration before SNI and after SNI in vehicle- or PEG-treated mice (n = 10 mice, one-way ANOVA, p < 0.001). (r) The effect of conditioned fear on phase 1 of the response to formalin-induced pain (n = 6 mice/group, unpaired two-sided t-test, n.s.) (s) Time spent licking paw (5 min bins) with control PBS or TMT in the chamber (n = 8 mice/group, two-way ANOVA, main effect of group p = 0.001, group x time interaction p = 0.0307). (t) Time spent licking paw during phase 1 (n = 8 mice/group, unpaired two-sided t-test, p = 0.0162). (u) TMT was applied to a chamber placed on 52 °C hot plate. Mice were then placed in the chamber and their behavior was recorded for 1 min. (v-x) Latency to lick the forepaw (v) and hind paw (w) and the number of jumps (x) on the hot plate after application vehicle or TMT (n = 9 mice/group (v), n = 10 mice/group (w), n = 8 veh, n = 9 TMT mice (x) unpaired two-sided t-tests, all n.s.). Data are expressed as mean ± SEM. Grey dots and lines represent individual mice. T-test and post-hoc comparisons: *p < 0.05, **p < 0.01, ***p < 0.001. ANOVA main effect of group: ##p < 0.01, ###p < 0.001. ANOVA interaction: †p < 0.05, †††p < 0.001.
