Fig. 3: Prolonged pain-related responses are suppressed by lPBN NPY–Y1R signalling during competing needs.
From: A parabrachial hub for need-state control of enduring pain
a, Experimental timeline. b, Time licking paw during phase 2 in ad libitum (ad lib.)-fed (n = 7) and food-deprived (FD) (n = 9) mice (unpaired two-sided t-test, P = 0.0025). c, Withdrawal threshold to mechanical stimuli in the CFA model (n = 15 mice; one-way ANOVA, P < 0.001). d, Withdrawal duration after acetone application in the SNI model (n = 8 mice; one-way ANOVA, P < 0.001). e, Experimental timeline. f, Time licking paw during phase 2 in mice with ad libitum water access (n = 8) and water-deprived (WD) (n = 6) mice (unpaired two-sided t-test, P = 0.0007). g, Withdrawal threshold to mechanical stimuli in the CFA model (n = 10 mice, one-way ANOVA, P = 0.0012). h, Withdrawal duration after acetone application in the SNI model (n = 11 mice; one-way ANOVA, P < 0.001). i, Experimental timeline. j, Time licking paw during phase 2 in mice with vehicle or TMT in the chamber (n = 8 mice per group; unpaired two-sided t-test, P = 0.0012). k, Experimental timeline. l, Time licking after formalin injection while animals were in either in the unshocked (n = 10) or the shocked context (n = 12) (unpaired two-sided t-test, P = 0.0495. m, A Y1R antagonist or vehicle was infused into the lPBN. n,o, Withdrawal threshold to cold (n; acetone) or mechanical (o) stimuli in ad libitum-fed (n = 11) or food-deprived (n = 8) mice with a vehicle or Y1R antagonist infusion. All mice had undergone SNI surgery 2 weeks prior (one-way ANOVA, P < 0.001). p,q, Withdrawal threshold to cold (p, acetone) or mechanical (q) stimuli in mice with ad libitum water (n = 15) and PEG-treated mice with a vehicle (n = 12) or Y1R antagonist (n = 9) infusion (one-way ANOVA, P < 0.001). All mice had undergone SNI surgery two weeks prior. r, Time licking paw during phase 2 in mice exposed to TMT (n = 7 vehicle, n = 8 Y1R antagonist mice; unpaired two-sided t-test, P = 0.0137). Data are mean ± s.e.m. Grey dots and lines represent individual mice.
