Extended Data Fig. 4: Gradual evaporation triggers loss of multicellularity in sheets from natural splash pool samples.
From: Clonal-aggregative multicellularity tuned by salinity in a choanoflagellate
Representative brightfield images of sheets identified in natural samples from Sp64 (Exped-A) over a 6-day gradual evaporation time course in the laboratory. After 48 h, sheets had disappeared and had been replaced by single cells appeared (black arrowheads). Time under gradual evaporation is depicted in the upper right. Salinity was not measured at 96 and 144 h. N = 2 independent replicates, two technical replicates each (n = 4). Figure related to Figs. 3 and 4.
