Extended Data Fig. 9: Aggregation is species-specific.
From: Clonal-aggregative multicellularity tuned by salinity in a choanoflagellate
(a) (top) Stills of C. flexa single cells labelled with CellTrace Far Red (magenta) and S. rosetta single cells labelled with CellTrace CFSE (green) 0 h post-dissociation. (bottom) Stills of C. flexa and S. rosetta 20-22 h post-dissociation. Note that C. flexa forms sheets composed of only C. flexa cells (magenta arrowheads), excluding S. rosetta cells (green arrowheads), which form chains clonally97. Unmixed C. flexa and S. rosetta cells were used as controls. (b) Quantification of percentage of C. flexa or S. rosetta cells in sheets in mixed conditions 20-22 h post-dissociation. N = 3 biological replicates, two technical replicates per condition (n = 211 sheets). p-value in b by the Mann-Whitney U test. Figure related to Fig. 6.
