Extended Data Fig. 3: Splash pools undergo natural cycles of evaporation and refilling.
From: Clonal-aggregative multicellularity tuned by salinity in a choanoflagellate
(a, c, e) Representative images of monitored splash pools near Boka Kalki (Sp15) and Boka Wandomi (Sp50 and Sp69) during a 9-day time course as part of Exped-A (n = 15 splash pools). Some splash pools experienced evaporation (a, n = 7), complete desiccation (c, n = 4), and evaporation and refilling (e, n = 4). Dashed lines: splash pool outlines. Upper left: salinity in ppt. Upper right: time in days. (b, d, f) Salinity (top) and depth (bottom) over time. Filled circles: active sheets observed. Empty circles: no sheets observed. Grey cross: one irregular non-motile sheet observed. Grey area: salinity saturation (outside the refractometer measurement range). Grey dashed lines in d and f: dry periods during which splash pools were completely desiccated. Orange circle in f: refilling events. Figure related to Fig. 3, Extended Data Figs. 1–2, and Table S1.
