Extended Data Fig. 8: Immunization of inactivated toxins and CspC is sufficient to protect against death but does not fully clear C. difficile.
From: Mucosal vaccination clears Clostridioides difficile colonization
a, Survival of WT C. difficile R20291 infection. b,c, Weight loss. Mean +/− SEM. d–g, Enumeration of total C. difficile R20291 bacteria (vegetative cells and spores) (d,e) and spores (f,g) in faeces. P value relative to RI dmLT-only control at the same time point (shown in Fig. 2). Individual lines correspond to individual mice. Limit of detection shown by dotted line, 500 CFU/g faeces. 8b-g: n = 10 per group, decreases in n due to animal mortality during infection. Weight loss and survival data for other groups taken from Fig. 2 (n = 10-15 per group). Statistical significance was calculated by means of [(a)] log-rank Mantel-Cox test, and [(b-g), (d-g, each day analysed individually)] one-way ANOVA with Tukey’s correction; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. RI, rectal instillation; d.p.i., days post-infection; CFU, colony forming units; NTAs, non-toxin antigens.
