Extended Data Fig. 7: Cryo-EM data processing and analysis of the SglCJ3–MurJ complex.
From: Convergent MurJ flippase inhibition by phage lysis proteins
a, Summary of Cryo-EM data processing workflow to obtain the SglCJ3–MurJ complex structure. b, Representative micrograph (left) and 2D class averages. c, Angular distribution of particles of the final 3D reconstruction. d, Fourier shell correlation (FSC) curved between two half maps. e, Local resolution map of the transmembrane region in the final Cryo-EM map colored by resolution from 3.2 Å (purple) to 4.4 Å (green). f, Cryo-EM density fit of the TM helices 1-14 of MurJ. All models are shown in sticks. Residues in MurJ making contacts with the BRIL domain are highlighted in orange.
