Supplementary Figure 9: Response of IRT1 + FER1 transgenic plants to culture in high-cadmium conditions. | Nature Biotechnology

Supplementary Figure 9: Response of IRT1 + FER1 transgenic plants to culture in high-cadmium conditions.

From: Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin

Supplementary Figure 9

Plants were treated with 10 μM CdSO4 (grey bars) twice a week for with a 0 μM treatment (white bars). (a) Leaf cadmium, (c) iron and (e) zinc concentrations of WT and IRT1 + FER1 transgenic plants grown at low and high cadmium. Storage root (b) cadmium, (d) iron and (f) zinc concentrations of WT and IRT1 + FER1 transgenic plants grown at low and high cadmium. Values are means of 4 biologically independent plants. Box and whisker plots were constructed using the R software package The upper whisker extends from the hinge to the largest value no further than 1.5* IQR from the hinge (where IQR is the inter-quartile range, or distance between the first and third quartiles). The lower whisker extends from the hinge to the smallest value at most 1.5* IQR of the hinge. Data beyond the end of the whiskers are called “outlying” points and are plotted individually. Statistical tests were two-sided using Student’s t-test compared to non-transgenic control. *, ** and *** represent significance levels at p ≤ 0.05, p ≤ 0.01 and p ≤ 0.001 respectively.

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