Supplementary Figure 11: Stake-derived VIT1, IRT1 + FER1 transgenic and nontransgenic control cassava plants grown under field conditions.

a) Iron, (b) Zinc concentration of storage roots (c) Shoot yield, (d) root yield, (e) number of roots and (f) harvest index from stake-derived VIT1 transgenic and non-transgenic control cassava plants grown under field conditions. (g) Iron, (h) Zinc concentration of storage roots (i) Shoot yield, (j) root yield, (k) number of roots and (l) harvest index from stake-derived IRT1 + FER1 transgenic and non-transgenic control cassava plants. Values are means of 20 biologically independent plants in 4 replicates (5 plants per replicate). Box and whisker plots were constructed using the R software package The upper whisker extends from the hinge to the largest value no further than 1.5* IQR from the hinge (where IQR is the inter-quartile range, or distance between the first and third quartiles). The lower whisker extends from the hinge to the smallest value at most 1.5* IQR of the hinge. Data beyond the end of the whiskers are called “outlying” points and are plotted individually. Statistical tests were two-sided using Student’s t-test compared to non-transgenic control. *, ** and *** represent significance levels at p ≤ 0.05, p ≤ 0.01 and p ≤ 0.001 respectively.