Supplementary Figure 15: Mineral retention of raw (white bars) and boiled (gray bars) VIT1 and IRT1 + FER1 storage roots.

Fe concentration after (a) boiling from storage roots of VIT1 transgenic cassava plants. (b) Fe and (c) Zn concentration after boiling from storage roots of IRT1 + FER1 transgenic cassava plants. For VIT1, n = 3 biologically independent plants and for IRT1 + FER1, n = 4 biologically independent plants (2 technical replicates/plant). Box and whisker plots were constructed using the R software package The upper whisker extends from the hinge to the largest value no further than 1.5* IQR from the hinge (where IQR is the inter-quartile range, or distance between the first and third quartiles). The lower whisker extends from the hinge to the smallest value at most 1.5* IQR of the hinge. Data beyond the end of the whiskers are called “outlying” points and are plotted individually. Statistical tests were two-sided using Student’s t-test compared to raw storage roots. *, ** and *** represent significance levels at p ≤ 0.05, p ≤ 0.01 and p ≤ 0.001 respectively.