Fig. 2: Storage-root mineral concentrations of VIT1 and IRT1 + FER1 transgenic cassava at harvest 12 months after planting under field conditions.

a,b, Iron (a) and zinc (b) concentrations in storage roots of VIT1 transgenic cassava plants. c,d, Iron (c) and zinc (d) concentrations instorage roots of IRT1 + FER1 transgenic cassava plants. For VIT1, n = 9 biologically independent plants (3 plants/replicate); for IRT1 + FER1, n = 4 biologically independent plants. Box-and-whisker plots were constructed with the R package ggplot2. The upper whisker extends from the hinge to the largest value, no further than 1.5× the IQR from the hinge. The lower whisker extends from the hinge to the smallest value, at most 1.5× the IQR of the hinge. Data beyond the ends of the whiskers are considered outlying points and are plotted individually. Statistical tests were performed with two-sided Student’s t test, relative to nontransgenic control. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.