Supplementary Figure 2: Molecular characterization of IRT1 + FER1 transgenic plants. | Nature Biotechnology

Supplementary Figure 2: Molecular characterization of IRT1 + FER1 transgenic plants.

From: Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin

Supplementary Figure 2

(a) PCR screening for presence of IRT1 and FER1 transgenes in leaves of 4-week old in vitro plants. Tubulin was used as a control. M- marker; WT- wildtype; EV- empty vector; N- negative water control; (b) Genomic DNA blot hybridization from wildtype and transgenic IRT1 + FER1 cassava leaves (6-week old) to determine T-DNA insertion number. Positive represents the plasmid DNA. Both gel and blots were cropped for better resolution. Quantitative expression of IRT1 and FER1 in (c, f) leaves, (d, g) fibrous roots and (e, h) storage roots of IRT1 + FER1 transgenic cassava plants. Tissues were collected from 16-week-old plants grown in the greenhouse. Expression was compared and normalized to protein phosphatase 2 (pp2A). Line 8023-2 expression values were adjusted to a value of 1 and all other expression values expressed relative to this line. Values are means of 4 biologically independent plants and error bars represent SD. This experiment was repeated 2 times independently with similar results.

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