Supplementary Figure 6: Genome editing results of Fix.
From: Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes
(a) Genotypes of the T0 transgenic plants of Fix. The letters A–D represent genes OSD1, PAIR1, REC8 and MTL, respectively. Upper case letters indicate no mutation, while lower case letters indicate frameshift mutations; lower case* indicates in-frame mutations. (b) Sequencing assay of mutations around the targets of OSD1, PAIR1, REC8 and MTL of Fix-#6, Fix-#10 and Fix-#22. The protospacer adjacent motif and target sequence of wild type (WT) are denoted in red and blue, respectively. The symbol - indicates deleted nucleotides.
